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------=_NextPart_01C92A36.31F80C30 Content-Location: file:///C:/08A8CE73/Differenziamentoemopoieticoinvitro-Prof.Zamai.pps_file/master07.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C92A36.31F80C30 Content-Location: file:///C:/08A8CE73/Differenziamentoemopoieticoinvitro-Prof.Zamai.pps_file/pres.xml Content-Transfer-Encoding: quoted-printable Content-Type: text/xml; charset="utf-8" ------=_NextPart_01C92A36.31F80C30 Content-Location: file:///C:/08A8CE73/Differenziamentoemopoieticoinvitro-Prof.Zamai.pps_file/slide0031.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Diapositiva 1
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Differenziamento emopoietico in vitro
<= /div>
Lori= s Zamai
Istituto di Istologia ed Analisi di Labortorio;
Istituto di Scienze Morfologiche,
Centro di Citometria e Citomorfologia,
Università di Urbino “Carlo Bo”
INFN LNGS Assergi, L’Aquila
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Università degli Studi di Urbino
“Carlo Bò”
3DLogoUniv
ASLEM
Ass.ne Sammarinese per la Lotta co= ntro le Leucemie e le Emopatie Mailgne
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Cellula = staminale
Aut= o-mantenimento
Div= isione Asimmetrica
Multilinearità (pluripotente)
capacità di dare ori= gine a più linee differenziative
Pla= sticità capacità di  differenziare in cellule diverse dal tessuto da cui sono originate e prelevate

Car= atteristiche delle Cellule Staminali
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Capacità di Automantenimento &#= 13;
Self Renewal Capa= city
Gl= obuli rossi
Piastrine
Linfociti
Monociti
Granulociti
Di= fferanziamento
nei diversi lineage
emopoietici 
(pluripotente)
Cellula Staminale Emopoietica
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<= /span>
nL’attività funzionale del sistema emopoietico è strettamente connessa alla sua peculiare organizzazione che prev= ede l’esistenza di quattro compartimenti principali:&#= 13;
ncompartimento delle cellule staminali
ncompartimento delle cellule progenitrici = (pluripotenti)
ncompartimento delle cellule immature (precursori orientati verso una sola linea emopoietica)
ncompartimento delle cellule mature
n
Organizzazione<= /b> del sistema emopoietico
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file:///C:/08A8CE73/Differenziamentoemopoieticoinvitro-Prof.Zamai.pps_file/slide0079.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Diapositiva 1
Emopoiesi e Citochine
La sopravvivenza, proliferazione e differenziamento della cellula staminale emopoietica sono regolate da citochine (proteine che come gli ormoni attraverso specifici recettori modulano l’attività funzionale di cellule e tessuti), classificate in quattro categorie: 1. quelle che inducono il reclutamento nel ciclo cellulare dei progenitori più primitivi, cineticamente e funzionalmente inerti (early acting growth factors, come FLT3-L e SCF) 2. fattori linea-non specifici che agiscono su progenito= ri = emopoietici di livello inter= medio (IL-3, GM-CSF) 3. fattori specifici per una linea maturativa, capaci di stimolare cellule già commissio= nate (G-CSF, M-CSF, EPO, TPO, IL-15). 4. fattori che agiscono in sinergia e potenziano l’azione dei primi = 3 fattori (IL-12, TGF-beta).=
------=_NextPart_01C92A36.31F80C30 Content-Location: file:///C:/08A8CE73/Differenziamentoemopoieticoinvitro-Prof.Zamai.pps_file/slide0082.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" Diapositiva 1
SCF, FLT3-L
FLT3-L
SCF, IL-3, GM-CSF
IL-7
G-CSF
GM-CSF <= span style=3D'position:absolute;top:57.75%;left:48.68%;width:9.17%;height:3.5%'= >IL-3
IL-7
IL-15
TNF-a
IL-4
GM-CSF <= span style=3D'position:absolute;top:85.75%;left:49.43%;width:9.17%;height:3.5%'= >IL-3
M-CSF
EPO
TPO
Emopoies= i e Citochine
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